Aim To study septic rats on changes in macrophage inflammatory protein-MiP-lα level in serum. Methods 60 SD rats were divided into two groups randomly:group A sham group,n = 30 and group B sepsis group,n = 30. In group A,appendix exploration was made while in group B,the animals received cecal ligation and puncture CLP. In each group,5 ml of saline was subcutaneously injected saline of 5 ml. All rats were taken to measure the levels of macrophage inflammatory protein-lα in serum through bronchial alvolar lavage fluid at 3,6,12,24,48 h. Results Compared with group A,the levels of MIP-1α in serum were increased significantly in group B(P ＜ 0.05). Conclusion The high levels of MIP-1α may be the causes of the damaged microcirculation and the elevated vascular may have a certain effect on the sepsis.%目的 研究脓毒症大鼠早期肺泡灌洗液巨噬细胞炎症蛋白-1α(MIP-1α)水平的变化及其与脓毒症的关系.方法 60只SD大鼠随机分成A组假手术组,n=30、B组脓毒症组,n=30,A组仅行盲肠探查术,B组采用盲肠结扎穿孔(CLP)制作大鼠脓毒症模型.两组术后即刻皮下注射生理盐水5 ml.各组均于术后3、6、12、24、48 h时点,留取肺泡灌洗液检测MIP-1α.结果 CLP术后3 h肺泡灌洗液MIP-1α即开始升高,明显高于A组(P＜0.05),且逐步升高,到术后12 h达峰值,随后逐渐下降,但在48 h时点仍然显著高于A组(P＜0.05).结论 脓毒症致急性肺损伤后MIP-1α水平明显升高,其升高可能是脓毒症导致急性肺损伤的原因之一,在脓毒症导致急性肺损伤发生发展中具有重要作用.
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