HPLC-ELSD法测定十味芪术通窍合剂中黄芪甲苷含量

摘要

目的 建立HPLC-ELSD法测定十味芪术通窍合剂中黄芪甲苷的含量.方法 采用Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,流动相∶乙腈-水(35:65),流速1.0 mL·min-1,ELSD参数:雾化温度36℃,漂移管温度90℃,气体压力35 psi.结果 黄芪甲苷进样量在3.08～24.60 μg范围内呈良好线性关系(r=0.999 7),平均加样回收率为98.5%(n=9).结论 该法操作简便,重复性好,灵敏度高,可有效控制十味芪术通窍合剂中黄芪甲苷含量.%Objective To establish an HPLC-ELSD method for determination of astragal-oside IV in shiweiqishutongqiao mixture. Methods Kromasil C18( 250 mm ×4. 6 mm,5 μm )Column was used. The mobile phase consisted of acetonitrile-water( 35:65 )and the flow rate was 1. 0 mL · min-1 . The ELSD parameters were atomization temperature 36℃ , drift tube temperature at 90℃ ,gas pressure 35psi. Results Astragaloside IV had a good linear relation in the range of 3. 08 ～ 24. 60μg( r = 0. 999 7 ). The average recovery( n = 9 )was 98. 5% . Conclusion This method is rapid,sensitive,and accurate, which can be used for the quality control of shiweiqishutongqiao mixture.