CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响

摘要

目的 研究CCL21能否参与诱导胃癌SGC-7901细胞发生上皮间质转化.方法 取不同浓度(0、50、100、150μg·L-1)的CCL21作用胃癌SGC-7901细胞,划痕试验检测该细胞侵袭能力.镜下观察该细胞形态的变化.Western blot分别检测E-cadherin、N-cadherin、Vimentin、MMP-9的蛋白表达.结果 150μg·L-1浓度的CCL21作用胃癌SGC-7901细胞后,较其它浓度侵袭能力强,同时使胃癌SGC-7901细胞的胞体逐渐变为长梭状,部分可见伪足生成.E-cadherin蛋白表达下降(P<0.05),N-cadherin、Vimentin、MMP-9蛋白表达升高(P<0.05).结论 CCL21可能参与诱导胃癌SGC-7901细胞发生上皮间质转化.%Objective To investigate whether CCL21 can induce epithelial-mesenchymal transition in gastric cancer cells SGC-7901. Methods SGC-7901 cells were induced by CCL21 in different concentration(0,50,100,150μg·L-1)and then the ability of inva-sion and metastasis was detected by scratch testing.Morphological changes in forms were observed.The protein expressions of E-cadher-in,N-cadherin,Vimentin,MMP-9 were detected by Western blot.Results After being induced by CCL21 in 150μg·L-1,SGC-7901 cells achieved faster ability of invasion and metastasis.SGC-7901 cells formed into a long spindle and pseudopod could be seen.The ex-pressions of N-cadherin,Vimentin,and MMP-9 rose(P<0.05)while that of E-cadherin obviously descended(P<0.05).Conclusions CCL21 may induce epithelial-mesenchymal transition in gastric cancer cell and promote the invasion and metastasis.