油污土壤降解细菌的分离鉴定及其生物强化研究

摘要

从大庆油田长期石油污染的土壤中分离出高效降解石油烃的菌株5种,通过生理生化分析和16SrD?NA全序列分析,初步确定为Pseudomonas sp.(假单胞菌属)、Pseudomonas aeruginosa(铜绿假单胞菌属)、Aci?netobacter junii(琼氏不动杆菌属)、Microbacterium oxydans(微杆菌属)、Pseudomonas aeruginosa(铜绿假单胞菌属).在摇瓶培养和土壤固体培养条件下的石油烃降解率都远远高出了对照组.同时通过改变初始盐浓度、N源和P源,初步探测了各菌株的最适生长和降解条件,为本土微生物强化的生物修复方法提供了新的菌种资源和信息,对进一步有效实现本土微生物强化具有重要意义.%5 bacterial strains were isolated from the long-term petroleum-contaminated soil in the Daqing oilfield which can efficiently degrade petroleum hydrocarbons. Using physiological and biochemical assays , as well as 16S rRNA sequencing analysis,we identified these strains as Pseudomonas sp.,Pseudomonas ae-ruginosa,Acinetobacter junii,Microbacterium oxydans,and Pseudomonas aeruginosa.Under both liquid and sol-id(i.e.soil)culture conditions,these bacterial strains showed much higher petroleum hydrocarbon degrading rates than the control strain. By modulating the concentration of initial salt,N and P in the medium,we ex-plored the optimal growth and degradation conditions of the bacterial strains. This study provides new bacte-rial resources and information for the autochthonous bioaugmentation of crude oil contaminated soil.