Due to differences in morphology, ecology and biology, the phylogenesis of Coniferopsida and Taxaceae remains controversial, and the phylogenetic relationship of Pseudotaxus is yet to be confirmed. Three rDNA regions, ITS1, 5.8S rDNA, and ITS2, were prepared for 77 species belonging to seven families of Coniferopsida, including Taxaceae. Maximum parsimony(MP)and Neighbor-joining(NJ) analyses were performed. Results showed that:(1) the Coniferopsida species formed three branches:(Taxaceae, Cephalotaxaceae, Cupressaceae, Taxodiaceae),(Araucariaceae, Podocarpaceae), and(Pinaceae);(2) Taxaceae included Taxus, Pseudotaxus, Amentotaxus, and Torreya; and(3) Pseudotaxus belonged to Taxaceae, with a support rate of 97%in MP analyses and 99% in NJ analyses.
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