马铃薯转 GhABF2转录因子苗耐盐性研究

摘要

In order to improve adaptability of crops to adversity ,transgenic potato seedlings with GhABF2 gene were employed as study materials to investigate their drought tolerance capability .Results showed that under the conditions of NaCl stress ,two lines with GhABF2 transgene displayed similar variations to the control plants in terms of dry weight , fresh weight ,physiological and biochemical indexes .Based on the phenotypic variations ,it was found that the transgenic plants were significantly strong in drought resistance .With the increase of NaCl concentrations ,compared with non-trans-genic plants ,biomass of two transgenic plants appeared to be significantly increased .In addition ,with the increase of NaCl concentrations ,chlorophyll content of all seedlings investigated became decreased ,whereas soluble sugar ,MDA , proline ,SOD activity ,and POD activity all appeared to be increased .Soluble protein content did not display significant variations .On basis of the plant growth status and its physiological and biochemical indexes , two transgenic plants showed stronger resistance than non-transgenic plants .%以转 GhABF2转录因子马铃薯试管苗为材料，进行苗期耐盐性研究，以期了解该作物对逆境的适应性。实验结果表明：在不同浓度NaCl胁迫条件下，转GhABF2基因株系材料（T1、T2）与对照未转基因材料（WT）植株的干鲜重、生理生化指标的变化趋势基本一致，就各性状值的变化来看，转基因植株表现出了明显的抗逆性。随着NaCl胁迫浓度的增加，与对照非转基因植物WT相比，转 GhABF2基因材料T1、T2的生物量显著增加；WT、T1、T2的叶绿素含量均随着盐浓度的增加而降低；可溶性糖、丙二醛、脯氨酸，SOD酶活性、POD酶活性均随着盐胁迫浓度的增加而升高；可溶性蛋白含量随着胁迫程度的加重略有下降，但变化趋势不显著。从植株的生长状态、生理生化等性状指标方面来看，转基因植株比对照非转基因植株表现出了更强的抗逆性。