Isolation and Identification of a Porcine Pseudorabies Virus Strain in Taizhou City

摘要

cqvip:In this study,the liver,kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District,Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus,which was named TAIZ130417. The growth titer of the isolated virus reached 108. 12 TCID50/ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies.