Optimization of Ultrasonic Extraction Conditions of Polyphenols from the Needles of Black Pine ( Pinus thunbergii ) Using Response Surface Methodology (RSM)

摘要

Ultrasonic extraction conditions of polyphenols from the needles of black pine ( Pinus thunbergii ) were optimized by single factors analysis and Box-Behnken experiment design. The results showed that both of ethanol concentration and ultrasonic time had a significant effect on polyphenol extraction, and there was significant interaction between any two variables in the three parameters of ethanol concentration, ultrasonic temperature and ultrasonic time. The optimal parameters for polyphenol extraction were the ethanol concentration of 52.67%, ultrasonic temperature at 49.21 ℃and ultrasonic time of 30.76 min. Under these conditions, the actual content of polyphenols was close to the predicted value. Therefore, the process can be used to extract polyphenols from black pine needles.