Optimization of the Sampling Method for Genetic Variation Survey in Maize Populations Using SSR Markers

摘要

In this study,single-plant DNA sampling method,multi-plant leaf-mixing DNA sampling method,and single-plant DNA-mixing sampling method were adopted,to analyze the genetic variations of Meng A population and C population 1 using SSR markers and establish the optimal technological system for analyzing genetic diversity of maize populations. DNA samples in different treatments were amplified using 34 SSR primers which were uniformly distributed in ten chromosomes of maize. Polyacrylamide gel electrophoresis was performed to analyze the polymorphism information content and genetic similarity coefficient of 60 individuals and compare the numbers of alleles amplified from DNA samples in different treatments. The results indicated that Meng A population and C population 1 both had relatively abundant genetic variations and the established technological system could be applied in researches of maize genetic diversity. Extracting DNA samples from mixed leaves of 12 individuals with five replications is the best sampling method,which could achieve similar results to mixed DNA samples of 12 individuals. This sampling method can be applied to analyze the genetic relationship among a large number of maize populations,which can not only reduce the workload,but also significantly improve the efficiency.