Study on Enzymatic Hydrolysis Technology and Antioxidant Activity of Glutinous Rice Glutinous Enzymatic Processes and Antioxidant Activity

摘要

[Objective] This study aimed to investigate enzymatic hydrolysis technology of glutinous rice and the oxidation resistance activity of the enzymatic hydrolysis solution. [Method] White glutinous rice was hydrolyzed using four kinds of proteases including neutral protease,alkaline protease,papain and trypsin.Using the scavenging rate of hydroxyl radical(·OH) as an indicator and appropriate protease as hydrolytic enzyme,the effects of protein substrate concentration,enzyme dosage,enzymatic hydrolysis temperature and initial pH on the abilities of proteases to scavenge hydroxyl radical from enzymatic hydrolysis solution of glutinous rice were investigated. Based on single-factor test,L9(34) orthogonal experimental design was adopted,to determine the optimal enzymatic hydrolysis conditions leading to the highest oxidation resistance activity of enzymatic hydrolysis solution. [Result] The optimized process parameters for enzymatic hydrolysis of glutinous rice protein with neutral protease were: protein substrate concentration of 2%,enzyme dosage of 24 000 U/g protein(protein meter),enzymatic hydrolysis temperature of 55 ℃,initial pH of 8. 0,and enzymatic hydrolysis duration of 0. 5 h; under these conditions,the hydroxyl radical scavenging rate could reach 56. 05%; protein substrate concentration,enzyme dosage,enzymatic hydrolysis temperature and initial pH had extremely significant effects on the hydroxyl radical scavenging rate. In addition,the activities of antioxidant peptides in glutinous rice hydrolysates were well maintained within a temperature range of 60-100 ℃.[Conclusion] The study produced theoretical feasibility reference for the production of functional base powder by spray drying.