Optimization of Polysaccharide Extraction from Radix Glehniae Root Bark by Response Surface Methodology and Anti-immunosuppressive Activity Analysis

摘要

[Objective] This study aimed to establish and optimize the extraction technology of polysaccharides from Radix Glehniae root bark,and to explore the processing methods of Radix Glehniae in producing areas. [Method] Based on the single-factor experiment,with polysaccharide content and polysaccharide yield as indices,various factors affecting polysaccharide yield from Radix Glehniae root bark were investigated by central composite design-response surface method. Moreover,immune functions of cyclophosphamide-induced blood-deficient mice in various polysaccharide groups were compared to investigate the effects of root bark removal of Radix Glehniae on medicinal quality. [Result] Ultrasonic extraction was the appropriate extraction technology of polysaccharides from Radix Glehniae root bark. The optimal ultrasonic extraction conditions were ultrasonic time 36 min,ultrasonic power 460 W,solid-liquid ratio 1∶ 10,ethanol concentration 80%,under which polysaccharide yield reached 16. 08%. Polysaccharides extracted from Radix Glehniae root( ALP) and Radix Glehniae root bark( BLP) could effectively improve immune function of blood-deficient mice with no significant differences. [Conclusion] Radix Glehniae could be used without removal of root bark.The optimized extraction process was stable and feasible,which laid a solid foundation for further comprehensive development and utilization of Radix Glehniae root bark.