Preparation of Polyclonal Antibodies against NS3 Protein of Japanese Encephalitis Virus

摘要

Japanese encephalitis(JE)is a central nervous system disease caused by Japanese encephalitis virus(JEV),which can infect human and a variety of animals and cause irreversible nerve damages.NS3 protein plays an important role in the process of JEV polyprotein hydrolysis,which is essential for JEV replication.Therefore,NS3 protein may be used as a potential drug target to treat Japanese encephalitis.In this study,the pET-28a-NS3 plasmid was successfully constructed and expressed in E.coli BL21(DE3)under IPTG induction.The molecular weight of the expressed recombinant protein was 55 ku,which was consistent with the expected result.The positive serum was prepared by immunizing BALB/c mice with NS3 protein and identified by indirect immunofluorescence(IFA).The results showed that there was a fluorescence reaction between the prepared positive serum of NS3 protein and cells infected with JEV.