离子对高效液相色谱法同时测定氧化型染发剂中5种染料

摘要

An ion-pair high performance liquid chromatographic method was established to simultaneous de-termination five dyes in oxidized hair dyes .The chromatographic conditions were as follows:Phenomenex kinetex C18 column ( 2.6μm, 4.6 mm ×150 mm ) was selected, with 10 mmol/L Octanesulfonic acid sodium solution ( pH 2.5 )-methanol ( 60∶40 , V/V) as the mobile phase , a diode array detector was used , detection wave-length at 280 nm, qualitative analysis by retention time and UV spectra characteristics , quantitative analysis by external standard method .Results showed good linearity for the 5 compounds ranging from 20 .0 mg/L to 100 mg/L, lowest detective limits for Quinhydrone is 0.02%, Resorcinol is 0.03%, o-aminophenol is 0.02%, o-phenylenediamine is 0.04%, 4-amino-2-hydroxytoluene is 0.03%.Average spiked recoveries of matrices at lower level were between 82.5%and 106%,with RSD between 1.4%and 6.1%.Average spiked recoveries of matrices at middle level were between 82.0%and 105%,with RSD between 0.4%and 2.2%.Average spiked recoveries of matrices at high level were between 78.5%and 105%,with RSD between 1.7%and 4.5%.%采用离子对高效液相色谱法同时测定氧化型染发剂中5种染料成分，选择Phenomenex kinetex C 18色谱柱（2．6μm，4．6 mm ×150 mm），以10 mmol/L辛烷磺酸钠水溶液（pH值2．5）＋甲醇（体积比60∶40）为流动相，采用DAD检测器，检测波长280 nm，以保留时间和紫外光谱特征定性，外标法定量。5种染料在20．0 mg/L～100 mg/L范围内线性关系良好，最低检出质量分数氢醌为0．02％，间苯二酚为0．03％，邻氨基苯酚为0．02％，邻苯二胺为0．04％，4-氨基-2-羟基甲苯为0．03％。低浓度基质加标平均回收率为82．5％～106％，RSD为1．4％～6．1％，中浓度基质加标平均回收率为82．0％～105％，RSD为0．4％～2．2％，高浓度基质加标平均回收率为78．5％～105％，RSD为1．7％～4．5％。