安徽省人乳头瘤病毒16型全基因组的结构特征

摘要

Objective To clone human papillomavirus type 16 genome from Anhui province, analyze genome se-quence of HPV16 and study its genomic characteristics. Methods Five pathological specimens of cervical cancer from Anhui province were collected and the total DNA was extracted. Specific primers were designed to clone HPV16 genome in four fragments. The sequence of four fragments was assembled manually and nucleotide sequence was analyzed after sequencing. Results A cervical pathological sample containing HPV16 was detected and the genome sequence with full length of 7 906 nts (GenBank accession number:KC935953) was obtained. Sequence alignment of genome nucleotide sequence showed that HPV16 of Thailand and HPV16 of Japan were more similar to HPV16 of Anhui (HPV16-Anhui) than other HPV genome nucleotide sequence, their similarity reached 99. 5%. Phylogeny tree analysis demonstrated that HPV16-Anhui and other 7 HPV16 clustered into a single branch. Con-clusion HPV16 genome nucleotide sequence is obtained from Anhui province for first time with great significance for further understanding of HPV16 variation from Anhui even east China.%目的克隆安徽省人乳头状瘤病毒16型( HPV16)全基因组,分析HPV16全基因组序列并了解其结构特征。方法收集5例安徽省宫颈癌病理组织标本并提取总DNA,设计4对特异性引物分段克隆HPV16全基因组,测序后进行序列拼接及核苷酸序列分析。结果检测到1个宫颈癌病理样本中含有 HPV16并获得全基因组序列,序列全长7906 nts ( GenBank登录号：KC935953)。序列比对显示,安徽HPV16( HPV16-Anhui )与泰国HPV16和日本HPV16的全基因组核苷酸序列相似性最高,达99.5%。系统关系树分析显示,HPV16-Anhui与其他7个HPV16型聚成1个单独的分支。结论获得安徽省首例HPV16全基因组核苷酸序列,HPV16-Anhui与不同地区 HPV16亲缘关系均较近且基因组变异性较小。