生长激素受体在宫腔粘连患者子宫内膜组织中的表达及意义

摘要

目的：探讨生长激素受体( GHR)在宫腔粘连( IUA)患者子宫内膜组织中的表达及意义。方法通过免疫组化MaxVision两步法和实时荧光定量PCR( qRT-PCR)法分别检测IUA患者(研究组)和非IUA患者(对照组)子宫内膜组织中GHR蛋白和 GHR mRNA 的表达情况。结果研究组GHR蛋白表达、GHR mRNA和对照组比较差异有统计学意义(P<0．05)。子宫内膜腺体与子宫内膜间质中GHR蛋白表达比较差异有统计学意义( P<0．05)。结论 IUA患者子宫内膜组织中GHR的表达低于非IUA患者，而且这种表达在子宫内膜腺体中较多，在子宫内膜间质中较少，为IUA患者在宫腔粘连分离术术后使用生长激素提供一定的理论参考。%Objective To investigate the growth hormone receptor ( GHR) expression and significance of endome-trial tissue in patients with intrauterine adhesions. Methods Immunohistochemistry MaxVision twostep and quanti-tative real-time PCR ( qRT-PCR) were used to detect the expression of GHR protein and GHR mRNA IUA in pa-tients endometrial tissue of IUA group ( study group) and non IUA group ( control group) . Results Using immu-nohistochemistry assay to detect GHR protein expression and qRT-PCR to detect the GHR mRNA expression, and the differences between the study group and the control group were statistically significant(P<0. 05). Immunohis-tochemistry was used to detect the expression of GHR protein in endometrial glands and endometrial stromal, the difference between the two parts were statistically significant ( P<0. 05 ) . Conclusion The expression of GHR in endometrial tissue in patients with IUA is lower than those of non-IUA, and this expression is more in endometrial glands, less in endometrial stroma,which provide a theoretical reference for the use of hormone in IUA patients after transcervical resection of adhesions( TCRA) .