振荡电场对脊髓损伤大鼠β-catenin蛋白表达和神经元再生的影响

摘要

Objective To explore the influence of oscillating field stimulation on expression of β-catenin protein and neurons regeneration in rat with spinal cord injury.Methods Sixty SD rats with spinal cord injury induced by Allen′smethod were randomly divided into experimental group and control group.The experimental group was implanted oscillating electrical field as intervention,while the control group just reveived oscillating electric field stimulator but not applied stimulus.At 3,7,14 d after experiment,Basso-Beattle-Bresnahan(BBB) Rating Scale was used to observe the recovery of motor function.Western blot,immunohistochemistry and immunofluorescence staining were used to analyze the expression of β-catenin protein and neurons regeneration of SD rats.The statistical analysis was performed between the two groups.Results BBB scores showed no significant difference at 3,7 d in two groups, while BBB scores presented a significant difference at 14 d(P<0.05).Western blot and immunohisto-chemistry results showed that the expression of β-catenin protein was higher than the control group at each time point(P<0.05),and the expression of β-catenin protein gradually decreased with the extension of the time in the two groups.Neurons expressed an increasing trend from 3 d to 14 d,while the number of neuron in the experimental group expressed better than that in the control group,and cell morphology was more regular.Conclusion Oscillating field stimulation can improve the expression of β-catenin protein and neurons regeneration in the injured spinal cord,which would contribute to neurological improvement after spinal cord injury.%目的 观察振荡电场对脊髓损伤大鼠β-catenin蛋白表达规律和神经元再生的影响.方法 60只SD大鼠采用改良Allen′s打击法建立脊髓损伤模型,随机分为对照组和实验组.实验组施加振荡电场干预,对照组只置入振荡电场刺激器而不给予干预.建模成功后分别在3、7、14 d取大鼠脊髓,行HE染色观察脊髓大体形态变化,采用Western blot和免疫组化技术检测脊髓中β-catenin蛋白表达规律,行免疫荧光检测观察神经元变化情况,并在取材前对两组大鼠行Basso-Beattle-Bresnahan(BBB)行为学评分.结果 Western blot和免疫组化结果均显示,3 d时两组脊髓灰质内β-catenin蛋白均高表达,随时间延长表达量逐渐减少,同时实验组在各时间点β-catenin蛋白表达量均高于对照组(P<0.05).神经元的表达在3~14 d时呈现递增的趋势,但实验组神经元在表达数量上明显优于对照组,同时细胞形态更加规则.两组BBB评分3、7 d时差异无统计学意义,而14 d时,实验组BBB评分明显高于对照组(P<0.05).结论 振荡电场可以提高大鼠脊髓损伤区域β-catenin蛋白表达量,并促进神经元再生,从而改善受损脊髓神经功能.