Early conversion stimulation method was used to analyze alkaloid composition and content in tobacco leaves of Dasuo 26 and their selfing populations in order to verify reliability and validity. Results showed that the selfing populations of plants with percent nicotine conversion ( PNC) lower than 2. 5% was stable and all had PNC lower than 2. 5% , indicating that the parent plants were non-converter. The three selfing generations from parent plants with PNC between 2. 5%-and 3% showed significant segregation of the trait of nicotine conversion within the range of 0. 439%-10. 331% , 0. 466%-21. 834, and 0. 394%-3. 757% , respectively, which indicated that the parents were converters. The selfing populations from parent plants with PNC greater than 3% showed great variation for nicotine conversion, and the 2 selfing lines from parents with PNC greater than 50% had the highest conversion level, indicating that the parents were high converters. Therefore, PNC greater than 2. 5% can be a reliable standard to determine a converter plant.%采用烟碱转化早期诱导方法,对白肋烟达所26及其自交后代不同烟株的烟碱转化率进行了分析测定,以确定烟碱转化株鉴别标准的可靠性和有效性.结果表明,烟碱转化率低于2.5％的烟株自交后代株系稳定,株间烟碱转化率变异小,且均低于2.5％,表明母代烟株为非转化株；烟碱转化率介于2.5％和3％的3个烟株自交后代均表现株间烟碱转化性状的分离,变异幅度分别为0.439％-10.331％、0.466％-21.834％和0.394％-3.757％,表明母代烟株为转化株.烟碱转化率大于3％的烟株自交后代烟碱转化率株间变异性大,烟碱转化性状分离严重；烟碱转化率大于50％的2个烟株自交后代烟碱转化率也都较高,是高转化株的表现.因此,烟碱转化率大于2.5％是确定转化株的可靠标准.
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