新鉴定高抗TMV普通烟草枯斑资源的N导入片段长度多样性

摘要

为了解我国普通烟草(Nicotiana tabacum)资源对烟草花叶病毒(Tobacco mosaic virus,TMV)的抗性基因状况,为烟草抗病育种的亲本选择提供信息,利用N基因特异分子标记鉴定出携带N基因的普通烟草资源,并采用N导入片段的特异分子标记鉴定了N导入片段的长度.结果表明,供试的63份接种TMV表现枯斑的普通烟草(简称普通烟草枯斑资源)可划分为3大类型,分别为Coker176类型:GL3.50和GL4.06标记阴性、TN4.39、TN5.226、TN5.51标记阳性;Samsun NN类型:GL3.50、GL4.06、TN4.39、TN5.226标记阳性,TN5.51阴性;Xanthi nc类型:GL3.50、GL4.06、TN4.39、TN5.226、TN5.51标记阳性.402等51份资源的N导入片段长度属于Coker176类型,Ky160等2份资源的N导入片段长度属于Samsun NN类型.Ky52等10份资源的N导入片段长度属于Xanthi nc类型.N导入片段较短的枯斑资源,推测其连锁累赘可能较小,育种利用潜力较大.本文较系统地鉴定了普通烟草抗TMV的枯斑资源的N导入片段长度,可为抗TMV种质资源的育种利用提供参考.%In order to understand the status ofNicotiana tabacum germplasm highly resistant to tobacco mosaic virus (TMV), to provide information for highly resistant parents selection in tobacco breeding, tobacco germplasm with N gene were selected by using N gene-specifi c molecular markers, and their N introgression segment lengths were tested by using N introgression segment specifi c molecular markers. Results showed that 63 tested tobacco germplasm could be divided into three main categories, Coker176 type: GL3.50 and GL4.06 marker negative, TN4.39, TN5.226, TN5.51 marker positive; Samsun NN type: GL3.50, GL4.06, TN4.39, TN5.226 marker positive, TN5.51 marker negative; Xanthi NC type: GL3.50, GL4.06, TN4.39, TN5.226, TN5.51 marker positive. The N introgression segment length of 402 and other 51 germplasm belonged to Coker176 type. The N introgression segment length of Ky160 and other 1 germplasm belonged to Samsun NN type. The N introgression segment length of Ky52 and other 9 germplasms belonged to Xanthi NC type. Germplasm with short N introgression segment may have less linkage drag, and are of greater breeding potential. The N introgression segment lengths of TMV highly resistant germplasm were evaluated in this paper, which could provide information for selecting highly resistant parents in variety development.