小花碱茅组织培养植株再生体系的建立

摘要

The effects of husk-off treatment and various hormone ratios on callus induction and differentiation were investigated using mature seeds of Puccinellia tenuiflora as explants.Finally,a plant regeneration system via tissue culture was established.The husk-off treatment increased seed germination rate and deceased con-tamination rate significantly.The optimal medium for callus induction was MS medium supplemented with 5.0 mg/L 2,4-D with the highest callus induction rate of 55.2%.The optimal medium for bud differentiation and rooting was MS medium supplemented with 1.0 mg/L 6-BA+0.5 mg/L NAA with the highest bud differentia-tion rate of 31.7%,the highest rooting rate of 81.7% within a period of two weeks.The rooting rate of regen-erated plants was up to 100% on half strength MS medium and their transplant survival rate in soil was also up to 100%.The whole process of plant regeneration via tissue culture lasted for about 16 weeks totally.The es-tablishment of plant regeneration system via tissue culture in P .tenuiflora has laid a foundation for further ex-ploring the molecular mechanism of its salt tolerance.%以小花碱茅成熟种子为外植体，研究了脱颖处理和不同激素配比对愈伤组织诱导和分化的影响，成功建立了其组织培养植株再生体系。结果表明，种子脱颖处理显著提高了种子发芽率，降低了污染率；诱导愈伤组织的最佳培养基为添加5．0 mg／L 2，4-D 的 MS 培养基，诱导率可达55．2％，2周可见白色愈伤组织；最佳芽分化培养基为添加1．0 mg／L 6-BA 和0．5 mg／L NAA 的 MS 培养基，分化率可达31．7％，同时伴随根毛的发生，生根率达81．7％，诱导时间为2周；分化后的再生苗移植于1／2MS 培养基上，100％生根，炼苗移栽后可全部成活。从小花碱茅种子诱导愈伤组织到植株再生共需16周左右。小花碱茅组织培养植株再生体系的建立为进一步探究小花碱茅耐盐碱分子机制奠定了基础。