优化的数字全息显微成像系统

摘要

Based on the analysis of the recording and reconstructing of hologram and the point spread function of pre-magnification digital micro-holographic system (PMDMHS), the performances of the six common PMDMHSs are compared in imaging resolution, imaging quality and conveniences, for the first time to our knowledge. The results show that the digital image-plane holography (DIPH) has the highest imaging resolution and the best imaging quality;the resolution of DIPH is independent of the photosensitive surface size of the recording device;this system can completely record the information about the object transmitted through the micro-objective (MO);it is not needed to consider the size of illuminated region of object. Moreover, the reconstruction process of DIPH is very simple. DIPH is an optimized digital holographic imaging system. The DIPH with equal curvature of reference wavefront and object wavefront is very conducive to phase unwrapping and phase aberration compensation;therefore DIPH is more suitable for phase microscopy. The experimental results demonstrate the validation of the theoretical analysis.%　　基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析，从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究。结果表明，像面数字全息术具有最高的成像分辨率及成像质量，其成像分辨率与记录器件的光敏面尺寸无关，该系统对信息的记录是完整的，而且记录过程不必考虑物体被照亮区域的大小，再现过程非常简单，是优化的数字全息显微成像系统。等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行，该系统更适合于位相显微。实验结果验证了理论分析的正确性。