INTRODUCTION Sea urchin is a marine animal of economic value. The gonad of Hemicentrotus pulcherrimus A. Agassiz and Anthocidaris crassispina A. Agassiz may be offered as food and medicine, the sea urchin shell may be used as fertilizer and artware and moreover, the sea urchin eggs are important materials for the studies in embryology and molecular biology. With the increasing exportation of sea urchins in China, the natural resources are gradually decreasing. In order to satisfy people’s growing needs of
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