[Objective] To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province. [Methods] Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences. [Results] Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences. In total 7 genera and 22 species were identified, including 46 strains in Gammaproteobacteria (51.1%) , 32 in Firmicutes (35. 6%) , 10 in Betaproteobacteria (11.1%) , and 2 in Alphaproteobacteria (2.2%). The dominant bacteria species were Pseudomonas and Bacillus. [Conclusion] There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang, and these bacteria may play a physical and ecological roles in nematodes.%[目的]了解黑龙江省大豆田大豆胞囊线虫胞囊可培养细菌的多样性.[方法]运用稀释平板法和16S rDNA基因序列的系统发育分析对胞囊可培养细菌多样性进行研究.[结果]用NA培养基从胞囊上分离90株具有不同菌落形态的细菌.16S rDNA序列分析结果表明:90株菌株分属于7个属22个种.46株属于变形菌门γ亚群(Gammaproteobacteria),32株属于厚壁菌门(Firmicutes),10株属于变形菌门β亚群(Betaproteobacteria),2株属于变形菌门α亚群(Alphaproteobacteria).假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属.[结果]黑龙江省大豆胞囊线虫胞囊中存在丰富的细菌物种多样性,这些细菌对大豆胞囊线虫可能具有一定的生理生态作用.
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