Objective To investigate the expression of Tenascin-C in human hepatocellular carcinomaCHCOand its role in HCC invasion,metastasis and angiogenesis. Methods Immunohistochemical staining was employed to detect the expression of Tenascin-C and microvessel density(MVD)in resected specimens of 54 cases of HCC,32 cases of hepatic cirrhosis and 17 cases of normal hepatic tissues. Reverse transcription polymerase chain reaction(RT-PCR) was used to detect Tenascin-C mRNA expression. Results The immunohistochemistry revealed that Tenascin-C was positively expressed in 34 cases of HCC and 6 cases of hepatic cirrhosis tissues,respectively^2 = 14. 06,P<0. 01). No Tenascin-C was detected in normal hepatic tissues. The im-munoreactivity of Tenascin-C in HCC was significantly higher than that in the cirrhosis tissues[(162. 15 + 9. 77)vs. (149. 24 + 8. 25) ,< = 2. 192 , P<0. 05]. The expression of Tenascin-C mRNA in HCC was also significantly higher than that in hepatic cirrhosis tissues[(0. 593 + 0. 110)to. (0. 138 + 0. 089) ,1 = 2. 894 ,P<0. 05]. There was a correlation between Tenascin-C expression and capsule invasionO = 2. 248,P<0. 05) ,Edmondson-Steiner grade(< = 2. 698,P<0. 05)as well as metastasis(< = 2. 785 , P<0. 01)in HCC. Additionally,MVD of HCC tissues was significantly correlated to the Tenascin-C expression(r = 0. 68,P< 0. 05). Forty-six cases of HCCC85. 2%)were followed up with a median period of 11. 5 months(range 3-30 months). The Te-nascin C-negative patients showed longer median survival time than the Tenascin-positive patients[(22. 01 + 6. 95)months vs. (15. 08 + 7. 06)months,P<0. 05,log-rank test]. Conclusion Up-regulation of Tenascin-C in HCC is possibly related to tumor invasion,metastasis as well as angiogenesis. Tenascin-C and MVD staining might be used to predict the metastatic and aggressive potential of HCC.%目的 研究Tenascin-C在原发性肝细胞癌(HCC)中的表达,探讨Tenascin-C与HCC浸润、转移和血管生成的关系.方法 采用免疫组织化学方法检测54例HCC、32例肝硬化、17例正常肝组织中Tenascin-C的表达情况以及微血管密度(MVD);采用逆转录聚合酶链反应(RT-PCR)方法检测Tenascin-C mRNA,并结合临床病理指标进行分析.结果 Tenascin-C在HCC、肝硬化和正常肝脏组织中的阳性例数分别为34、6和0,其差异具有统计学意义(χ2=14.06,P＜0.01);Tenascin-C在HCC组织内的表达强度明显高于肝硬化组织[(162.15±9.77)vs.(149.24±8.25),t=2.192,P＜0.05].HCC组织Tenascin-C mRNA的表达强度明显高于肝硬化组织[(0.593±0.110)vs.(0.138±0.089),t=2.894,P＜0.05].有包膜浸润的HCC组织中Tenascin-C表达量明显高于无浸润者(t=2.248,P＜0.05),Edmondson-Steiner病理分级中HCC属Ⅲ～Ⅳ级的Tenascin-C含量明显高于Ⅰ～Ⅱ级者(t=2.698,P＜0.05),HCC有转移的Tenascin-C含量明显高于无转移者(t=2.785,P＜0.01).HCC组织中Tenascin-C表达与微血管密度(MVD)呈正相关(r=0.68,P＜0.05).获随访的46例(85.2%)HCC患者在随访期间(3～30个月,平均11.5个月),Tenascin-C阴性表达的患者中位生存时间长于Tenascin-C阳性表达的患者[(22.01±6.95)月vs.(15.08±7.06)月,P＜0.05,log-rank test].结论 Tenascin-C对HCC的浸润、转移和血管生成起着重要作用.Tenascin-C和MVD可以作为预测HCC浸润、转移的生物学指标.
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