表没食子儿茶素没食子酸酯对活性氧的抑制作用

摘要

Reactive oxygen species (ROS)overproduction could cause oxidative stress,leading to oxidative damage of lipids,DNA and protein,and also triggering a series of physiological and pathological reactions.(-)-Epigallocatechin-3-gallate (EGCG)is the main component of polyphenols in green tea,which has a strong antioxidant activity and could ef-fectively inhibit the generation of ROS.In this paper,the origination and physiological role of ROS,and the characteris-tics of EGCG and its inhibited effects on ROS will be introduced.In addition,the quenching effects of EGCG on singlet oxygen (1 O2 )generation during photosensitization of Rose Bengal (RB)in aqueous solution has been investigated by the direct detection of 1 O2 luminescence at 1 270 nm,the quench effects of EGCG and sodium azide (NaN3 )for 1 O2 was comparatively analyzed.It was concluded that the quenching effects of EGCG on 1 O2 was superior to NaN3 ,provi-ding a theoretical basis for quantitative study of EGCG quenching effects on 1 O2 .%生物体内的活性氧（Reactive oxygen species，ROS）过量引起氧化应激将导致脂质、DNA 和蛋白质氧化损伤，从而引发一系列生理和病理反应。绿茶中茶多酚的主要成分表没食子儿茶素没食子酸酯（（-）-Epigallo-catechin-3-gallate，EGCG）具有强抗氧化性，能有效抑制 ROS。本文简要介绍了生物体内 ROS 的来源和 EGCG 的特性及其对 ROS 的抑制作用。通过检测玫瑰红水溶液在光敏化时所产生1 O2的1270 nm 近红外发光，分析比较了 EGCG 和迭代钠（NaN3）对1 O2发光的淬灭过程，发现 EGCG 对1 O2的淬灭效果比 NaN3更好，为 EGCG 淬灭1 O2的定量研究提供理论依据。