多尺度的光声显微镜用于癌细胞及实体瘤成像

摘要

多尺度显微成像系统(M-PAM)被发展,并被用于成像从癌细胞到实体肿瘤的多尺度生物结构.该装置由二维运动平台,扫描振镜,物镜,聚焦超声换能器组成,其横向分辨率达到3 μm.结果显示该系统可以对体外培养黑色素瘤细胞与体内的黑色素瘤进行无标记成像.基于具有靶向性的探针,M-PAM系统可以对体外培养的U87-MG肿瘤细胞以及体内U87-MG实体肿瘤进行成像.综上所述,M-PAM系统将是研究肿瘤的有力工具.%A multiscale-photoacoustic microscopy (M-PAM) system is developed for imaging living biological structures ranging from cancer cells to solid tumors.It integrates a two-dimensional motor platform,a two-dimensional scanning galvanometer,an objective lens,a focused ultrasound transducer.The lateral resolution of the M-PAM is quantitatively analyzed to be 3 μm.The results demonstrated that the melanoma cells in vitro and melanoma in vivo were revealed by this system with label-free.With the assistant of targeted photoacoustic probes,M-PAM can image U87-MG tumor cancer cells in vitro and U87-MG solid tumors in vivo.M-PAM will be a powerful tool for tumor study ranging cells to solid tumor in vivo.