人支气管上皮细胞16HBE和其经硫化镍、反式-BPDE诱导的恶性转化细胞对脂质体转染效率的差异性比较

摘要

目的:通过脂质体法转染不同荧光蛋白质粒到多种接种密度细胞的效率差异探讨恶性转化细胞的癌变过程.方法:以16HBE、结晶型NiS诱导的16HBE恶性转化细胞和反式-BPDE诱导的16HBE恶性转化细胞为实验对象,采用不同的细胞接种密度,用脂质体法对它们分别转染两种不同的表达荧光蛋白的质粒,通过荧光显微镜观察细胞的转染情况.结果:无论转染eGFP质粒还是GFP-LC3质粒,在多种细胞密度下,正常细胞16HBE都比恶性转化细胞的转染效率低.结论:恶性转化细胞在癌变过程中可能发生了细胞膜通透性的改变.%Objective: To investigate the carcinogenesis of malignant transformation cells by comparing the difference in transfection efficiency of exogenous plasmids through liposome between normal cells and malignant transfected cells. Methods: Two types of plasmids that express the fluorescent proteins were transfected with liposome into cells such as 16HBE and its malignant transformation cells induced by crystalline nickel sulfide and anti-benzo (a) pyrene separately, and the transfection were observed by fluorescent microscope. Results:In a range of cell density, the transfection effi-ciency of normal cells 16HBE is lower than that of the malignant transfected cells both with the plasmids eGFP and GFP-LC3. Conclusion:Inconsistency in membrane permeability between normal cells and malignant transfected cells leads to inconsistency in transfection efficiency.