高原低氧适应动物新疆灰旱獭右心室重构的组织学改变

摘要

目的：探讨新疆灰旱獭高原低氧适应性改变致右心室重构组织学改变。方法应用免疫组化技术检测新疆灰旱獭右心室缝隙连接蛋白43（CX43）蛋白表达，同时应用HE染色和Masson染色观察心室肌结构和纤维化程度变化。结果心肌细胞肥大，胶原纤维增多，右心室肥厚指数、体重指数明显增高。 CX43蛋白表达减少和（或）分布的改变。结论高原低氧致新疆灰旱獭右心室结构重构，可作为研究高原低氧适应性机制的理想动物模型。%Objective To investigate the histological changes of the right ventricular remodeling in Xinjiang Mar-mota baibacina which are adapted to high-altitude hypoxia .Methods Hematoxylin and eosin and Masson staining was used to examine the pathological changes and degree of fibrosis , and immunohistochemical technique was used to detect connexin 43 ( CX43) expression in the right ventricular myocardium .Results In the right ventricular myocardium , cardio-myocyte hypertrophy and increased amount of collagen fibers were observed .The right ventricular hypertrophy index and weight index of Marmota baibacina were significantly higher , and expression of CX43 was significantly reduced than that of normal rats ( P<0.05 for both ) .Conclusions High-altitude hypoxia induces right ventricular remodeling in Xinjiang Marmota baibacina.It can be used as an ideal animal model for studies on the mechanisms of hypoxia adaptation on high -altitude plateau .