中药加味理肠饮对腹泻型肠易激综合征大鼠SP、VIP、CORT含量的影响

摘要

目的:观察加味理肠饮对腹泻型肠易激综合征(IBS-D)大鼠SP、VIP、CORT含量,以及病理学变化的影响.方法:将SD大鼠随机分为7组,除空白对照组外,各组均予避水应激+夹尾刺激+番泻叶灌胃方法建立IBS-D大鼠模型,后分别给予相应药物灌胃,连续用药28 d后处死大鼠,运用Elisa法检测大鼠SP、VIP、CORT含量,采用HE染色检测大鼠结肠组织病理学变化.结果:与空白组对比,模型组大鼠SP、VIP、CORT均上调(P<0.05);与模型组对比,除中药低剂量组外,各药物组SP、VIP、CORT均下调(P<0.05),尤以中剂量组明显(P<0.01);各实验组大鼠的结肠组织均未见明显的形态学改变.结论:加味理肠饮中剂量组能够有效的治疗IBS-D,其作用机制可能与调节SP、VIP、CORT表达有关.%Objective:To observe the effect of modified Jiawei Lichang Yin on the contents of SP, VIP and CORT in IBS rats of diarrhea type( IBS-D) and its influence to pathological changes of colon. Methods: IBS-D model was estab-lished by the method of water avoidance stress( WAS) , tail clamping and Folium Sennae intervention. The normal rats were taken as the blank control. Contents of SP, VIP and CORT were tested by Elisa method, and the pathological changes of rats' colon by HE dying after 28 days of medication. Results:The levels of SP, VIP and CORT were signifi-cantly increased after modeling compared to those of the control group ( P <0 . 05 ); they were down -regulated after treatment in all medication groups, except for the low-dose TCM group(P<0. 05);of which, the middle-dose TCM group showed the most obvious change(P<0. 01). There was no obvious morphological changes in the colon tissue in IBS-D rats after medication. Conclusion:The middle-dose of modified Jiawei Lichang Yin is effective for IBS-D, which mechanism is related to regulating the expressions of SP, VIP and CORT.