利用GUS组织化学染色法,研究由拟南芥泛素蛋白连接酶HECT家族基因(UPLs)启动子起始的β-葡萄糖苷酸酶报告基因(GUS)的表达模式.结果表明:UPLs家族中6个成员的启动子在拟南芥植株的不同组织和不同时期均有所表达,且在莲座叶发育的早期和衰老期表现出较高活性.进一步观察up l3和up l4突变体发现,up l3和up l4突变体除了up l3有较明显的叶型变化,两者均表现为延迟衰老现象;且突变体中衰老相关基因的表达水平不同于野生型.研究推测UPL3和UPL4可能参与了叶片生长发育过程的调控,同时还参与调控植株衰老进程.%In this research ,we use GUS histochemical staining method to analyze the expression pattern of GUS gene of ProUPLs : GUS transgenic plant ,which initiate the GUS report gene by the promoter of HECT E3s genes (UPLs).The results of these six HECT E3s genes at different development stages and tissues show different expression patterns ,but all of them have high activity at the early stage and senes-cence stage of leaf development .Interestingly ,we find that the leaf shape of upl3 mutant show significant difference and both up l3 and up l4 mutant delay senescence and have different expression levels of senes-cence-associated genes compared to wild type .Thus ,we speculate that both UPL3 and UPL4 are likely to regulate the leaf development and the progress of plant senescence .
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