选取258份国内外烤烟品种,在2个试验点调查13个农艺性状,利用16对引物组合获得的597个SRAP标记进行分子遗传多样性和群体结构分析,选取适宜的模型进行农艺性状与SRAP标记间关联分析.结果表明,参试品种表型和基因型变异丰富,国内品种的遗传多样性低于国外品种.总材料可分为7个亚群,分类结果与材料的地理来源和遗传背景显著相关,国外材料遗传结构相对复杂.通过比较发现MIM_Q+K和MIM_PCA+K为该群体最优的关联分析模型,共检测到18个SRAP标记与6个农艺性状显著关联(-logP＞2,P＜0.01),其中,me1/em9-16、me1/em9-36、me4/em9-1与株高,me1/em2-14、me7/em5-34与叶片数,me6/em2-6与脚叶宽,me2/em6-15与腰叶长在不同环境下均显著关联.%To assess the genetic diversity of flue-cured tobacco germplasm, developing the elite gene resources, we selected 258 flue-cured tobacco varieties from China and abroad to investigate 13 agronomic traits in two locations with different environments. The genetic diversity and population structure were analyzed with 597 SRAP markers produced by 16 primer combinations. Different association models were tested and the most effective model was chosen for the population to make the association analysis between agronomic traits and SRAP markers. The results revealed that abundant phenotypic and genetic diversity existed in the flue-cured tobacco germplasm accessions. Flue-cured tobaccos from China showed less diversity than those from other countries. The accessions could be divided into seven subpopulations and the classification result was significantly correlation with geographic origins and hereditary relationship. Population structure within exotic varieties was multiplex. MIM_Q+K and MIM_ PCA+K were effective models for association analysis between agronomic traits and SRAP markers. A total of 18 markers were found to be significantly associated (-log P>2, P<0.01) with six agronomic traits. Among them, markers associated with plant height (mel/em9-16, mel/em9-36, me4/em9-l), leaf number (mel/em2-14, me7/em5-34), bottom leaf width (me6/em2-6) and lumbar leaf length (me2/em6-15) were stably detected in different environments. This study provides evidence for identification of elite flue-cured tobacco germplasm, prediction of candidate gene and marker-assisted selection.
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