静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究

摘要

采用实时荧光定量PCR(qRT-PCR)技术对宁夏地方优良品种静原鸡的肝脏、肌肉、心脏、睾丸、卵巢、腹脂6个组织中ELOVL2和ELOVL5基因的表达量进行了测定,并通过SAS8.2软件进行分析统计.结果表明,ELOVL2和ELOVL5基因在静原鸡6个不同组织中均有表达.其中ELOVL2基因在肝脏中的相对表达量最高,为71.52±0.41,显著高于其他组织(P0.05).ELOVL5基因在静原鸡不同组织中的相对表达量依次为:肝脏>腹脂>卵巢>睾丸>心脏>肌肉,同样在肝脏中的表达量最高,为110.94±0.02,显著高于其他组织(P0.05).对ELOVL2和ELOVL5基因分别在不同组织中的表达差异进行分析比较,发现两个基因在腹脂中的表达量差异显著(P0.05).The relative expression of ELOVL5 gene in different tissues of Jingyuan chicken was as followed: liver>abdominal fat>ovary>testicle>heart>muscle.The highest expression level in liver was 110.94±0.02, which was significantly higher than that in other tissues (P0.05).The expression of ELOVL2 and ELOVL5 genes in different tissues was analyzed and compared.The results showed that the expression of two genes in abdominal fat was significantly different (P<0.05), and the expression of ELOVL5 gene in abdominal fat was higher.The research results showed that there were differences in the expression of ELOVL2 and ELOVL5 genes in six tissues from Jingyuan chicken, and liver tissue was the most obvious, which lays the foundation for the development of local technology platform to build local varieties of molecular breeding and genetic evaluation of germplasm resources.