葡萄鲨烯合成酶基因的克隆与序列分析

摘要

应用RT-PCR和RACE技术,克隆获得了葡萄鲨烯合成酶基因(SQS)的全长cDNA(1595 bp).序列分析结果表明:该基因包含1个完整的1242 bp开放阅读框,编码413个氨基酸残基；葡萄SQS与三七(Panax notoginseng)、人参(Panax ginseng)、甘草(Glycyrrhiza uralensis)的SQS分别有84％、83％、84％的一致性.%The full -length cDNA (1595 bp) of squalene synthase gene (SQS) in Vitis vinifera was cloned by RT - PCR and RACE here. Sequence analysis showed that this gene contained a complete open reading frame of 1242 bp encoding 413 amino acid residues. The SQS gene in Vitis vinifera showed 84% , 83% , 84% similarity with its homology in Panax notoginseng, Panax ginseng, Glycyrrhiza uralensis, respectively.