添加生物炭对烤烟碳氮代谢的影响

摘要

In order to identify the effects of biochar on carbon and nitrogen metabolism of flue-cured tobacco, taking the to-bacco variety( Nicto ianatabacumL, K326) as material, conducted the systematic research from the cell tissue structure, gene ex-pression and metabolism in three levels.Results showed that the starch were more at the earlier stages, and degraded more com-pletely in the treatment of no applying biochar with leaf maturity.The analysis of gene expression displayed that the expression of the genes related to carbon metabolism increased gradually after topping, while the expression of the genes related to nitrogen me-tabolism reduced.The expression of the genes were higher by applying more biochar at the later stages.The analysis of substance contents indicated that the contents of total sugar and reducing sugar reduced after applying biochar, the contents were higher after cured.The starch contents were higher after applying 600 kg/hm2 .The contents of total nitrogen and nicotine increased after ap-plying biochar, the difference was diminished after cured, these results showed that the metabolism of tobacco carbon and nitrogen were affected by applying biochar, thus affected the quality of flue-cured tobacco.%为探究添加生物炭对烤烟碳氮代谢的影响，以烟草品种K326为材料，从细胞组织结构、基因表达以及物质代谢3个层面进行了系统研究。超微结构分析表明：中部叶成熟早期，不施生物炭处理的烟叶淀粉粒积累较多，随着叶片成熟降解更快。基因表达分析显示：打顶后，糖、淀粉代谢相关基因表达逐渐增强，氮代谢相关基因的表达减弱。中部叶成熟后期，添加生物炭越多，相关基因表达越强。物质代谢分析表明：打顶后，添加生物炭处理的总糖、还原糖含量降低，调制后，糖含量较高。打顶及调制后，添加600 kg／hm2生物炭处理的淀粉含量较高。添加生物炭后，总氮、烟碱含量增加，调制后差异变小，这说明添加生物炭对烟叶碳氮代谢有较大影响，进而影响烟叶品质。