辣椒细胞质雄性不育系和保持系线粒体DNA差异的SRAP分析

摘要

SRAP ( Sequence-related amplified polymorphism) was analyzed between mitochondrial DNA of cytoplasm male sterility line 21A and its maintainer line 21B of pepper. The analysis showed that a total of 1 440 amplified fragments and nine polymorphic fragments between 100-1 000 bp were detected by applying 128 SRAP primer combinations; the polymorphic fragment percentage was 0.63% ; seven of the polymorphic fragments was detected in male sterility line 21 A. The polymorphic fragments were isolated, cloned and sequenced, blast of the 9 clones in GenBank showed that it all have homologous with energy metabolism genes. According to the sequences, three specific primers were designed to transform the SRAP marker to more stable SCAR markers,and they only detected in the 21A line,this suggested that the newly detected fragments related to cytoplasm male sterility.%以辣椒细胞质雄性不育系21A及其保持系21B的线粒体DNA为材料进行SRAP分析,结果表明,从128对引物扩增获得了1 440条100 ～1 000 bp的条带,多态性位点仅有9个,占0.63％,其中7条多态性条带位于21A中；对多态性条带回收、克隆和测序分析后发现,9个克隆在GenBank中找到了相似的功能,绝大部分与能量代谢有关；利用21A中的多态性序列特点设计SCAR引物,对21A和21B的基因组DNA进行扩增验证,3对引物在21A中扩增出目的条带,表明已成功地将SRAP标记转化为SCAR标记,是新发现的与辣椒细胞质雄性不育基因相关的片段.