Supercharged Natural Killer Cells Preferentially Expand CD8 + Cytotoxic T Cells While Targeting CD4+ Helper T Cells

摘要

NK mediated expansion of T-cells preferentially expands CD8+ but not the CD4+ subsets in both healthy and cancer donors. This results in a lower CD4/CD8 ratio. While NK Cells target CD4+ helper T-cells they do not target their CD8+ cytotoxic counterparts. This study also reveals that Cancer patients NK cells have reduced cytotoxic ability and secrete much less IFN-gamma than healthy donors and Osteoclast and sAJ2 expanded super-charged NKs intended for immunotherapeutic use from healthy donors expand more immune cells at a faster rate. NK immunotherapy was studied in combination with supplementation of probiotic bacteria in the humanized mouse model. NK immunotherapy increased CD8+ cytotoxic T cell population in immune tissue compartments and Increased immune infiltration in the tumor microenvironment, while probiotic supplementation further enhanced these effects, resulting in decreased tumor burden. This novel expansion technique can provide a non-invasive and highly effective treatment for the treatment of oral squamous cell carcinoma and other tumorigenic cancers.