首页> 外文学位 >Characterization of late blight (Phytophthora infestans) resistance of potato breeding lines with the RB gene from Solanum bulbocastanum.
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Characterization of late blight (Phytophthora infestans) resistance of potato breeding lines with the RB gene from Solanum bulbocastanum.

机译:利用马铃薯鳞茎的RB基因鉴定马铃薯种系的晚疫病抗性(Phytophthora infestans)。

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摘要

Late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most important disease of potato (Solanum tuberosum L.) because it affects foliage, tuber yield and storage ability. Previous work demonstrated that the wild diploid potato species S. bulbocastanum is highly resistant to all known genotypes of P. infestans. In this study, we transformed and expressed the RB gene from S. bulbocastanum (Rpi-blb1) into conventionally bred, late blight resistant breeding lines to evaluate the effect of pyramided late blight resistance genes. All RB potato transformation events were confirmed by Polymerase Chain Reaction (PCR) and RB expression was detected by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Foliar host plant resistance was characterized using detached leaf bioassays (DLB), greenhouse whole plant bioassays (WPB) and an inoculated field trial. A set of four P. infestans genotypes (US-8, US-22, US-23 and US-24) were used to evaluate foliar resistance in the DLB and WPB experiments, whereas only the US-22 genotype was used in the field. The results of the greenhouse whole-plant bioassays and detached leaf bioassays were varied. However, the highest level of resistance was demonstrated in conventionally bred, late blight resistant lines, which also contained the RB gene from S. bulbocastanum. The results from the field trial were more explanatory and proved that pyramiding resistance genes is an effective strategy to increase the level of late blight resistance.
机译:晚疫病(晚疫病)引起的晚疫病是马铃薯(Solanum tuberosum L.)最重要的病害,因为它影响枝叶,块茎产量和储存能力。先前的工作表明,野生二倍体马铃薯种球茎葡萄球菌对所有已知的致病疫霉基因型具有高度抗性。在这项研究中,我们将鳞茎链球菌(Rpi-blb1)的RB基因转化并表达到常规育成的晚疫病抗性育种系中,以评估金字塔状晚疫病抗性基因的作用。通过聚合酶链反应(PCR)确认所有RB马铃薯转化事件,并通过逆转录酶-聚合酶链反应(RT-PCR)检测RB表达。使用离体叶片生物测定法(DLB),温室全植物生物测定法(WPB)和接种的田间试验来表征叶面宿主植物的抗性。在DLB和WPB实验中,使用了一组四种感染的P. infestans基因型(US-8,US-22,US-23和US-24)来评估叶片抗性,而在现场仅使用US-22基因型。 。温室全植物生物测定和离体叶片生物测定的结果各不相同。然而,在常规育种的晚疫病抗性品系中显示出最高水平的抗性,该品系还包含鳞茎链球菌的RB基因。田间试验的结果更具解释性,证明了抗金字塔基因是提高晚疫病抗性水平的有效策略。

著录项

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Plant sciences.
  • 学位 M.S.
  • 年度 2014
  • 页码 75 p.
  • 总页数 75
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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