Applications of gluco-oligosaccharide oxidase

摘要

The nascent study of carbohydrate-active enzymes is now influencing a variety of industries at the commercial scale, particularly in processing lignocellulosic biomass for the production of fuels, materials, and other commodities. These enzymes are highly specific and can modify sugars with selectivity unmatched by chemical processes. Our group has worked extensively with gluco-oligosaccharide oxidase (GOOX), and feel the enzyme's oxidative activity would prove desirable in many new and existing applications where other commercialized carbohydrate oxidases are employed. The work presented here has demonstrated the improved characteristics of an immobilized fusion enzyme, CtCBM22A_GOOX-Y300A, on insoluble polysaccharide fibre, with potential applications in food additives, protein purification, and aldonic acid production. A second study has successfully employed GOOX oxidation as part of a synthetic pathway to generate a clickable monomer from glucuronoxylan capable of forming a carbohydrate-based polytriazole polymer; a process which could be extended to include neutral hemicelluloses such as galactoglucomannan.