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Investigating the effects of modified oil synthesis genes on carbon and nitrogen partitioning in Arabidopsis thaliana.

机译:研究修饰的油合成基因对拟南芥中碳和氮分配的影响。

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摘要

In recent decades, there has been a dramatic shift in the balance of the natural carbon cycle brought upon by increased anthropogenic greenhouse gas emissions (GHG); most notably, carbon dioxide (CO2), Biofuels may playa significant role in the preservation of the environment by providing a means of GHG mitigation, as well as a cleaner, renewable energy source.;Results showed dramatic changes in the progression though specific developmental stages among the S-5 and S-6 genotypes, as well some changes in normal C and N partitioning. It was also observed that the microbial ecology of the surrounding rhizosphere was altered resulting from genetic modification.;In this experiment, knock-down mutants of Arabidopsis thaliana expressing reduced seed oil content (S-5 ∼25.77%; S-6 ∼24.89%; AS11∼31.23%) were used to determine whether genetically modified oil synthesis genes affected carbon (C) and nitrogen (N) partitioning within the plant. Molecular analyses of bacterial communities in the rhizosphere were also conducted.
机译:近几十年来,由于人为温室气体排放量(GHG)增加,自然碳循环的平衡发生了巨大变化。最显着的是二氧化碳(CO2),生物燃料可通过提供减少温室气体的手段以及一种更清洁的可再生能源在保护环境中发挥重要作用。 S-5和S-6基因型之间的差异,以及正常C和N分区的某些变化。还观察到由于基因改造而改变了周围根际的微生物生态学;在该实验中,拟南芥的敲除突变体表达了降低的种子油含量(S-5〜25.77%; S-6〜24.89% ; AS11〜31.23%)用于确定转基因油合成基因是否影响植物中碳(C)和氮(N)的分配。还进行了根际细菌群落的分子分析。

著录项

  • 作者

    Fitzner, Christopher.;

  • 作者单位

    Saint Mary's University (Canada).;

  • 授予单位 Saint Mary's University (Canada).;
  • 学科 Biology Plant Physiology.
  • 学位 M.Sc.
  • 年度 2010
  • 页码 134 p.
  • 总页数 134
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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