TAF1 regulation of gene expression: Genome-wide localization and transcription profiling.

摘要

The general transcription factor II D (TFIID) nucleates the assembly of general transcription factors (GTF) and RNA polymerase II at the promoters to form the pre-initiation complex (PIC). TBP-associated factor I (TAF1), the scaffold of TFIID, not only interacts with other factors to stabilize the protein complex but also has several enzymatic activities.;In this study, genome-wide ChIP-on-chip analysis revealed that TAF1 recognizes and binds in vivo to more than 1,800 core promoters. From the transcription profiling, 4,531 genes have been identified with more than a 1.25 fold change in the transcriptional level, including 2,780 genes with reduced transcription and 1,751 with an enhanced transcription level. Among TAF1 target genes, 53% of genes were up-regulated by TAF1, especially the cyclins and cell cycle regulators. Genes such as members of the heat shock protein family were associated direct with TAF1 down-regulation. This study suggests that TAF1 is an essential transcription factor and is involved in various biological processes including cell cycle progression and the stress response.