Phylogeographic analyses of obligate and facultative cave crayfish species on the Cumberland Plateau of the southern Appalachians.

摘要

Cave systems and their unique biota are widely viewed as highly endangered, yet very little is known about basic life history, ecology, distributions, habitat requirements, and evolutionary relationships of subterranean species. The crux of the problem in cave studies is the assumption that traditionally defined morpho-species represent distinct evolutionary lineages. Convergence is exhibited in the morphologies of many animal groups, vertebrate and invertebrate, which leads to confusion in diagnosing species' boundaries, geographic distributions, gene flow routes, and imperilment. This dissertation research includes phylogeographic analyses of freshwater cave-dwelling crayfishes in the Southern Appalachians, a global hotspot of subterranean biodiversity. By examining population structure in light of habitat, geology, geography, and hydrology, we can better provide conservation direction for these groundwater species. Chapter one introduces a method, Nested Clade Phylogeographic Analysis (NCPA), used to investigate hypotheses about historical and current population structures within species.; Chapter two examines two competing hypotheses regarding conservation status of cave-dwelling species using a wide-ranging group of obligate subterranean crayfish species on the Cumberland Plateau's western escarpment. Using a population genetic approach, cave crayfish exhibited moderate to high levels of genetic diversity and attained large population sizes over their evolutionary histories.; Chapter three explores phylogeography and habitat differences within the facultative cave-dwelling crayfish species Cambarus tenebrosus . This freshwater species is unique in that it inhabits surface and subsurface karst environments, has an unusually large distribution, and exhibits troglomorphism with reduced eyes and elongated limbs.; Chapter four examines the obligate cave crayfish assemblage, genus Cambarus, subgenus Aviticambarus, which ranges across the southernmost area of the Southern Appalachians, which is known to contain the highest species diversity of obligate terrestrial animals in the United States. (Abstract shortened by UMI.)