Structural genomics of Fragaria: Wild and cultivated strawberries.

摘要

The extensive phenotypic variability and complex genetic makeup of the cultivated strawberry Fragaria x ananassa permits advances in plant improvement, a factor breeders have exploited to great benefit. However, the introgression of specific characters is complicated due to the cumbersome genetics and limited knowledge of genome structure and function of genes relevant to traits of interest. The present study represents the first genomics-level insight into strawberry genome structure and explores the hypothesis that a new type of molecular marker, the Gene-Pair Haplotype represents a transferable marker that may hasten linkage mapping in the diploid and octoploid strawberry.; My research presents the findings of four related research activities. First, an efficient and unified method for genomic DNA isolation was derived from over 100 experimental tests and conditions. Next, 1% of the Fragaria genome was sequenced and functionally annotated, using a bioinformatics approach and computational tools. Over 120 kb of intergenic regions were sequenced using the Gene-Pair-Haplotype approach, allowing for some initial relationships to be formulated concerning the diploid subgenome contribution to octoploid strawberry. Finally, Gene-Pair Haplotypes were used to add a suite of alleles to the growing Fragaria linkage map. These findings provide a starting point for further analyses of the strawberry genome.