Chapter 1 Introduction
1.1 The research background of the project
1.1.1 Interindividual variabilities in drug metabolism
1.1.2 CYP450 enzyme family
1.1.3 Breast cancer
1.1.4 CYP4Z1
1.1.5 Promoter and transcription factors
1.2 The purpose and significance of the projects
1.2.1 Missense Mutations in Human CYPs and UGTs
1.2.2 Promoter analysis of human CYP4Z1 encoding gene
Chapter 2 Materials and Methods
2.1 Missense mutations in human CYPs and UGTs
2.1.1 Gene list
2.1.2 Genetic variants from exome sequences and bioinformatic filtering
2.2 Promoter analysis of human CYP4Z1 encoding gene
2.2.1 Search of target transcription factors binding sites
2.2.2 Construction of reporter plasmids
2.2.3 Transfection of recombinant vector into MCF-7 and MCF-10A
2.2.4 Detection of Nano-luminescence
Chapter 3 Results and Discussion
3.1 Human CYPs
3.1.1 CYP1 family
3.1.2 CYP2 family
3.1.3 CYP3 family
3.1.4 CYP4 family
3.1.5 CYP5, 7 and 8 families
3.1.6 CYP11 family
3.1.7 CYP17, 19, 20, 21 and 24 families
3.1.8 CYP26 family
3.1.9 CYP27 family
3.1.10 CYP39, 46 and 51 families
3.2 Electron-donating redox partners in CYP systems
3.3 Human UGTs and UGDH
3.3.1 UGT1 family
3.3.2 UGT2 family
3.3.3 UGT3 and 8 families
3.4 Analysis of the CYP4Z1 promotor
3.5 Nano-luciferase activity measurements
3.5.1 CYP4Z1 promoter deletion analysis with first batch of fragments
3.5.2 CYP4Z1 promoter deletion analysis with second batch of fragments
3.5.3 CYP4Z1 promoter deletion analysis with third batch of fragments
Chapter 4 Conclusions and Prospect
4.1 Influence of 83 gene reference sequences redefinition
4.2 Conclusion and future prospect of CYP4Z1 promoter analysis
参考文献
Notes on publications and participation in scientific research
致谢
天津大学;
