论文说明
声明
Table of Contents
Abstract
摘要
List of abbreviations
List of Figures
List of Tables
Introduction
Chapter 1 Review of Literature：Probiotic concept and evolution
1．1．Probiotics
1．2．Nutritional and therapeutic purposes of probiotics
1．3．Probiotics in animal nutrition
1．4．Probiotics mechanisms of action
1．5．L．acidophilus ssp
1．6．Probiotic and Chronic diseases
1．7．L．acidophilus Utilization
1．2．1．Probiotics side effects
1．2．2．Means for Classification of S．cerevisiae
1．2．3．Study of the effect of probiotics on body weight and lipid metabolism
References
Chapter 2 Review of Literature：Selenium
2．1．Selenium
2．2．Mechanisms of action
2．3．Metabolism of Selenium
2．4．The Physical and Chemical Forms of Selenium
2．5．The physical propriety of selenium
2．6．Chemical Forms of Selenium
2．7．Sources of Selenium
2．7．1．In Soils
2．7．2．Plant Sources
2．8．Selenium supplementation
2．9．Serum selenium and oxidative balance
2．10．Antioxidant and lipid profile in obese mice
2．10．1 Superoxide Dismutase(SOD)
2．10．2．Superoxidase Dismutase Source
2．10．3．Malondialdehyde(MDA)
2．10．4．Catalase(CAT)
2．10．5 Catalase reaction
2．10．6．Selenium side effects
2．11．Study of the effect of selenium-enriched on body weight，lipid metabolism and gene expression
References
Chapter 3 Review of literature：Obesity and Lipid metabolism
3．1．Introduction
3．1．1 Lipid metabolism
3．1．2 Symptoms
3．1．3．Lipoproteins
3．1．4．High-density lipoproteins(HDL)
3．1．5．Low-density lipoproteins(LDL)
3．1．6．Aspartate aminotransferase(AST)／(ALT)
3．1．7．Serum total protein
3．2．Obesity and lipid gene expression
3．2．1．Cholesterol synthesis
3．2．2．The fatty acid oxidation pathway in mitochondria
3．2．3．Fatty acid synthesis(FAS)
3．2．4．Carnitine palmitoyltransferase Ⅱ(CPT2)
3．2．5．Acyl eoenzyme A oxidase(ACOX2)
3．2．6．Acetyl-CoA acetyltransferase(ACAT2)
3．2．7．Lipoprotein lipase(LPL)
3．2．8．Peroxisome proliferator-activated receptor(PPAR)
3．2．9．Sterol Regulatory Element-Binding Proteins(SREBPs)
3．3．Hepatic steatosis
References
Chapter 4 Effects of selenium——enriched probiotics on lipid metabolism，antioxidative status，histopathological lesions and biochemical indices in mice fed by high fat diet
4．1．Introduction
4．2 Material and methods
4．2．1．Preparation of Complex Probiotie
4．2．2．Experimental design
4．2．3．Sample Collection
4．3．Results
4．3．1．Body performance，liver weight and liver ratio
4．3．2．Gross findings
4．3．3．Histopathological examination
4．3．4．Serum levels AST，ALT，TP，TC，TG，LDL-C and HDL-C
4．3．5．Discussion
References
Chapter 5 Effects of selenium-enriched probiotics on antioxidant enzyme activities of SOD，CAT，GSH-Px and MDA level
5．1．Introduction
5．2．Material and methods
5．2．1．Experimental Procedure
5．2．2．Animals feeding
5．2．3． Statistical analysis
5．2．4．Results
5．2．5．Discussion
Refefences
Chapter 6 Effects of Selenium-enriched on mRNA lipid gene expression
6．1．Introduction
6．2． Material and methods
6．2．1．Experimental Procedure
6．2．2．Liver collection
6．2．3．mRNA Extraction and Real-Time PCR Assay
6．2．4 Statistical analysis
6．3．Results
6．4．Discussion
References
Summary
New knowledge
Dedication
Research implication
Acknowledgements
