GENOME--WIDE IDENTIFICATION OF LIPOXYGENASE GENE FAMILY IN COTTON AND FUNCTIONAL CHARACTERIZATION OF GbLOX5 IN RESPONSE TO V.dahliae INFECTION

目录

声明

Contents

Abstract

摘要

List of abbreviations

Chapter 1：Review of literature

1．1 The plant immune system

1．2 Importance of cotton in response to abiotic and biotic stresses

1．2．1 Effect of salinity stress on cotton growth and production

1．2．2 Effect of V．dahliae infection on cotton growth and production

1．3 Plant oxylipins

1．3．1 Research on Plant Lipoxygenase

1．3．2 Functions of LOX-derived oxylipins

1．3．3 Functions of LOXs in plant growth and development

1．3．4 Functions of LOXs in response to abiotic stress

1．3．5 Functions of LOXs in disease resistance

1．4 Aims and overview of the present study

Chapter 2：Genome-wide identification of lipoxygenase gene family in cotton and functional characterization in response to abiotic stresses

2．1 Introduction

2．2 Materials and methods

2．2．1 Isolation and sequence retrieval of LOX gene family from four species of cotton

2．2．2 Phylogenetie and subcellular localization analysis

2．2．3 Genes structure，conserved motifs and promoter analysis

2．2．4 Plant material and treatment for expression analysis

2．2．5 Vector construction and procedure for VIGS in cotton

2．2．6 Plant material and salt tolerance assays for functional analysis of GhLOX genes

2．2．7 Determination of salt stress-related physiological parameters

2．2．8 qRT-PCR analysis

2．3 Resuits

2．3．1 Genome-wide identification of LOX gene family in Gossypium spp

2．3．2 Phylogenetic analysis of LOX gene family in cotton

2．3．3 Structural and Motif analysis of GhLOX genes

2．3．4 Cis-elements analysis of GhLOX genes

2．3．5 Tissue specific expression patterns of GhLOX genes

2．3．6 Expression analysis of GhLOX genes under different abiotic stresses

2．3．7 Expression patterns of GhLOX genes under different exogenous phytohormone treatments

2．3．8 Silencing of GhLOX12 and GhLOX13 compromises cotton tolerance to salt stress

2．4 Discussion

Chapter 3：Functional characterization of GbLOX5 in response to V．dahliae infection

3．1 Introduction

3．2 Material and methods

3．2．1 Vector construction and plant transformation

3．2．2 Cotton transformation and growth of transgenie plants

3．2．3 DNA extraction and PCR for the selection of positive plants

3．2．4 Gene expression analysis

3．2．6 Southern blotting

3．2．7 Growing of seedfings，fungus preparation and inoculation experiments

3．2．8 Determination of Lignin contents

3．3 Results

3．3．1 Cloning and sequence analysis of GbLOX5

3．3．2 Phylogenetic analysis

3．3．3 Expression pattern analysis of GbLOX5

3．3．4 Genetic transformation and acquisition of transgenic plants

3．3．5 Cotton 9-LOX，GbLOX5 contributes to susceptibility in response to V．dahliae infection in cotton

3．3．6 GbLOX5 is a negative regulator of JA synthesis and signaling pathway in response to V．dahliae inoculation and treatment of MeJA

3．3．7 Lignin metabofism varies among silenced and overexpressed lines of GbLOX5 upon V．dahliae inoculation in cotton

3．4 Discussion

References

Appendix

Related Publications

Acknowledgements