The functional study of miRNA 142-3p in Haematopoiesis using zebrafish

摘要

MiRNAs are small non-coding RNAs involved in fine-tuning of gene regulation.MiRNAs are short, about 21 nucleotides in length, non-coding, regulatory RNA molecules representing a new layer in post-transcriptional gene expression regulation.MiRNA 142-3p has important role in erythropoiesis and its regulation so in our study we try to find exactly miRNA 142-3p role in the regulation of erythropoiesis and find miRNA142-3p target in Haematopoiesis.Over expression of miRNA142-3p caused significantly decrease in erythropoiesis and blood formation but in the same time miRNA-142 knockdown embryos had significantly fewer myeloid progenitor cells and expanded erythroid progenitor cells, and this confirms that miRNA 142-3p essential role to determination of myelo-erythroid progenitor cell fate in vivo.

目录

封面

声明

英文摘要

目录

1 INTRODUCTION

1.1 MiRNA:

1.2 Expression of miRNAs in the Cardiovascular System:

1.3 Role of miRNAs in Cardiovasculer Development:

1.4 MiRNA and Human blood cancers:

1.5 The role of Meis1 in normal Hematopoiesis:

1.6 Zebrafish \(Danio rerio\) as a model animal:

1.7 Objective:

2 MATERIALS AND METHODS

2.1 Experimental Animals and embryos collection:

2.2 Total RNA Isolation:

2.3 Meis1-3UTR cloning:

2.4 Single digestion:

2.5 Constructs design and mRNA synthesis of meis1:

2.6 mRNA synthesis of meis1-egfp: Capped Transcription Reaction As-sembly:

2.7 Microinjection confirmation meis1-egfp:

2.8 O-dianisidine staining:

2.9 Microinjection of miRNA 142-3p:

3 RESULTS

3.1 MiR 142-3P is expressed during zebrafish early developmental stage:

3.2 MiR-142-3P is required for erythoid formation repression and mye-loid expansion in zebrafish:

3.3 MiR-142-3p represses meis1 expression in zebrafish:

4 DISCUSSION

致谢

参考文献