DETECTION OF HEPATITIS E VIRUS IN GREATER CAIRO TWO WASTEWATER TREATMENT PLANTS AND ITS PREVALENCE AMONG WORKERS OF THIS PLANTS

摘要

Because of inadequate public sanitation, epidemics of HEV infection have beenrnreported in several developing countries. HEV- specific cDNA was prepared byrnreverse transcription of the total RNA extracted from water samples. Specified DNArnamplification by PCR demonstrates the presence of HEV in sewage samples from therninlets (PCR positive 10/36 : 27.77 %), while outlet samples were PCR negative. HEVrnIgM was detected in 40 workers out of 78 in these two studied plants (age 20-60rnyear), with percentage of 51.25 %, and HAV IgM was also detected in 3 workers outrnof 78 (3.84 %). The study of serum ALT and albumin level go with the increase in HEVrnIgM in sera. This study which was carried out in two different wastewater treatmentrnplants showed that HEV contamination was higher in one of them (EL-Berka) thanrn(EL-Zenin). A total of 33.33 % of influent water samples were positive and 55.25 % ofrnworkers were HEV IgM positive in the first plant (EL-Berka) and the correspondingrnfigure in the other plant (EL-Zenin) showed lower contamination, 22.22 % in influentrnwater sample and 40.9 % of workers.