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Determination of Gallic Acid in Longan Fruits by High Performance Liquid Chromatography

机译:高效液相色谱法测定龙眼果实中的没食子酸

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摘要

High performance liquid chromatography (HPLC) method was used to determine the content of gallic acid in the different parts of longan fruits. HPLC chromatograms with very good separate effect were obtained. The method was simple and quick with the high coefficient of correlation,delicacy,recovery and repeatability in detecting gallic acid. The gallic acid content in fruit of six longan (Dimocarpus longan Lour.) cultivars grown in Fujian province showed difference. The content of gallic acid in different parts of the fruit was the following sequence: seed>pericarp>aril. The content of gallic acid in seed was about 4.6-7.4 and 5.7-10.5 times higher than that of pericarp and aril,respectively. In seed, 'Lidongben' had the highest gallic acid content and 'Dongbao' had the lowest ones. In pericarp,'Fuyan' and 'miaoqiao' had the highest gallic acid content and 'Dongbao' had the lowest ones.In aril,'Miaoqiao' and 'Lidongben' had the highest gallic acid content and 'Dongbao' had the lowest ones.
机译:采用高效液相色谱法测定龙眼果实不同部位的没食子酸含量。获得了具有很好分离效果的HPLC色谱图。该方法简便快捷,检测没食子酸的相关系数高,精密度高,回收率高,重复性好。福建省六个龙眼(Dimocarpus longan Lour。)品种果实中的没食子酸含量存在差异。果实不同部位的没食子酸含量依次为种子>果皮>茴香。种子中没食子酸的含量分别约为果皮和假种皮的4.6-7.4倍和5.7-10.5倍。在种子中,“李冬本”的没食子酸含量最高,而“东宝”的没食子酸含量最低。果皮中,“富烟”和“苗桥”的没食子酸含量最高,而“冬宝”的没食子酸含量最低;在假种皮中,“苗桥”和“利东本”的没食子酸含量最高,而“东宝”的没食子酸含量最低。

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