Effect of Spleen Deficiency Syndrome on the Expression of TLR2 and TLR4 in Rats

摘要

[Objective]The aim of the paper was to study the expression of TLR2 and TLR4 in the rat's spleen deficiency syndrome (SDS) induced by reserpine. [Method]Twelve SD rats were divided into the control group and SDS group. After 7 days, the rats were sacrificed. The duodenum, jejunum and ileum was removed and washed in phosphatebuffered saline (PBS). One part of each intestine sections was fixed and paraffin embedded. The TLR2 secretion were observed by immunohistochemical staining. The other part was to measure the expressions of TLR2 and TLR4 were measured using RT-PCR. [Result]The results indicated that comparing with control group, the TLR2 secretion of SDS group was significantly decreased. The expression of TLR2 and TLR4 of SDS group in jejunum were significantly decreased (P < 0. 01), but TLR4 in ileum was increased (P < 0. 01). [Conclusion]In conclusion, these results indicated that SDS induced damage in jejunum, which resulted in the down-regulation of the expression of TLR2 and TLR4.