Multispectral Endoscopy and Microscopy Imaging Using a Spectrally Programmable Light Engine

摘要

We report a spectrally and temporally programmable light engine based on a spatial light modulator that candynamically create any narrow or broadband spectral profile for hyperspectral, fluorescence, or principal componentimaging. Most hyperspectral or multispectral imaging systems use wavelength selection devices such as acousto-optictunable filters (AOTFs), tunable grating or prism-based monochromators, or filter wheels. While these devices canselect wavelengths they cannot create arbitrary spectral profiles. This simple and economical system can be controlledat high speed (up to 5000 illumination profiles per second). Digitally controlled illumination is bit additive with imagedata providing high dynamic range imaging with monochrome or color imaging devices. This is especiallyadvantageous for endoscopes employing small well CCD or CMOS sensors since the dynamic range now can extendbeyond the limits of the sensor itself. In this report we show multispectral images of in vivo tissue and in vitro tissuesamples using endoscopes, surgical microscopes and conventional microscopes.