Fibronectin And Vitronectin Interactions With Naoh-treated Plga Increases Chondrocyte Functions

摘要

We have previously demonstrated that NaOH-treated poly(lactic-co-glycolic acid) (PLGA) scaffolds enhanced articular chondrocyte (cartilage synthesizing cells) adhesion, proliferation, and subsequent functions such as glycosaminoglycan production. Understanding the mechanisms behind this phenomenon requires exploring the involvement of initial protein adsorption events that provide a link and communication between the substrate surface and the cell. Substrate-dependent protein conformational changes have been shown to control cell proliferation and differentiation. Specifically, for this study, we concentrated on two important proteins for mediating chondrocyte functions: fibronectin and vitronectin. The initial interactions (such as adsorption and conformation) of these proteins were determined on NaOH-treated and non-treated PLGA scaffolds in an attempt to understand increased functions of chondrocyte on Na-OH treated PLGA.